Journal: bioRxiv
Article Title: The anterior cruciate ligament in murine post-traumatic osteoarthritis: markers and mechanics
doi: 10.1101/2021.11.08.467455
Figure Lengend Snippet: A-B) The ACLs of healthy control knee joints and PTOA knee joints (B) where analysed at the tibial enthesis (black box) and midbody (yellow box) regions. C-E) Collagen type II (COL2) expression was present in the fibrocartilaginous tibial enthesis of the healthy control ACL and in the tibial enthesis and mid-body regions of the PTOA ACL (black arrows). F-H) SOX9 chondrogenesis transcriptional factor expression was also found in the tibial enthesis and mid-body regions of the PTOA ACL (black arrows). I-K) RUNX2 hypertrophic transcriptional factor was expressed in the tibial enthesis region of the PTOA ACL (black arrows). L-N) Asporin (ASPN), a small leucine-rich proteoglycan, was expression in the tibial enthesis of the control ACL and extended within the mid-substance of the PTOA ACL. Scale is 25um for immunohistochemistry (higher magnification), and 50um for A-B (lower magnification).
Article Snippet: Slides were then blocked for endogenous peroxidase with 0.3% hydrogen peroxide (Sigma, 15 minutes), for endogenous Avidin/Biotin binding with an Aviding/Biotin Blocking Kit (Vector Labs, SP2001; 15 minutes each), and for non-specific binding sites (COL2: blocking solution from MoM Kit, Vector Labs, BMK-2202; SOX9, RUNX2, ASPN: 10% v/v goat serum).
Techniques: Expressing, Immunohistochemistry